Sir Alex Ferguson admitted that full-backs Patrice Evra and Rafael were tired after the 2-2 draw with Chelsea last Sunday, but are not expected to be rested after nearly a week off between games.
Darren Fletcher (bowel condition) will miss the remainder of the season, while Nani (hamstring), Paul Scholes (knee) and Phil Jones (ankle) are all doubtful and will undergo late fitness tests.
Ahead of his first match in charge of Reading's first team, caretaker boss Eamonn Dolan has a few injury concerns to contend with ahead of the trip to Old Trafford.
Goalkeeper Adam Frederici (ankle) looks set to be out until the end of March and he is joined by Jimmy Kebe (groin) and Pavel Pogrebnyak (three-match suspension).
Jason Roberts (hip) continues to miss out, but Alex McCarthy has been back in training and looks to be touch-and-go due to a lack of match fitness.
- Following Brian McDermott's (pictured right) sacking, academy coach Eamonn Dolan will take charge of his first match for Reading's senior side on Saturday.
The Royals have not beaten Manchester United since 1927 when they triumphed over the Red Devils in the FA Cup.
Reading come into this match on the back of five consecutive defeats in all competitions.
The Royals have already lost twice to United so far this season,
losing 4-3 at the Madejski Stadium in the league and 2-1 at Old
Trafford in the FA Cup.
United have come out with three points in 14 of their last 16 Premier League matches.
The only team to beat the hosts in their last 20 fixtures is Real Madrid.
- Reading have completed the least amount of passes in the league with 69 per cent meeting the intended target.
Sir Alex Ferguson's side, on the other hand, have completed 85.7% of their passes, a stat bettered only by Arsenal.
Robin van Persie has scored just one goal since the end of January, coming against Everton over a month ago on February 10.